Journal: Cell Death & Disease

Article Title: Oncogenic GALNT5 confers FOLFIRINOX resistance via activating the MYH9/ NOTCH/ DDR axis in pancreatic ductal adenocarcinoma

doi: 10.1038/s41419-024-07110-w

Figure Lengend Snippet: A–C Colony formation assays evaluating the proliferation difference of Patu8988 ( A ) and SW-1990 ( B ) experienced RNAi of GALNT5 treated with or without FOI (Three fields assessed per dish, three individual biological replicates performed, Two-way ANOVA, *** p < 0.001). FOI: the combination of 5-FU (4um), oxaliplatin (10um), and Irinotecan (10um) based on IC50 in WT PDAC cells, respectively. D Flow cytometry revealing the apoptosis difference of Patu8988 and SW-1990 experienced RNAi of GALNT5 treated with or without FOI (Three individual biological replicates performed, Two-way ANOVA, *** p < 0.001). ( E-F ) Flow cytometry revealing the cell cycle phase alteration difference of ( E ) Patu8988 and ( F ) SW-1990 experienced RNAi of GALNT5 treated with or without FOI (Three individual biological replicates performed, Two-way ANOVA, *** p < 0.001). G–J Oxaliplatin dose-response relationship curves were drawn and IC50 of oxaliplatin in the GALNT5 knockdown cell line ( G ) PATU8988 and (H)SW1990 and overexpression cell line ( I ) MiaPaca-2 and ( J ) PANC1 were calculated. K–N Oxaliplatin dose-response relationship curves were drawn and IC50 of oxaliplatin in the GALNT5 knockdown cell line ( K ) PATU8988 and ( L ) SW1990 and overexpression cell line ( M ) MiaPaca-2 and ( N ) PANC1 were calculated. Q–R statistics analysis of IC50 differences demonstrating IC50 alterations of (O-P) oxaliplatin and Q – R Irinotecan when GALNT5 was knocked down or overexpressed. (Three individual biological replicates performed, Two-way ANOVA, ** *p < 0.001). S–T IC50 alteration fold change showing differences between degrees of oxaliplatin and Irinotecan IC50 in cell lines treated with GALNT5 knockdown and overexpression. (Three individual biological replicates performed, Two-way ANOVA, *** p < 0.001).

Article Snippet: FOI is the combination of 5-fluorouracil (10uM), oxaliplatin(8uM), and irinotecan (8uM) in vitro experiments based on IC50 in WT PDAC cells respectively, and Concentrations of 5-fluorouracil (23 mg/kg), oxaliplatin(10 mg/kg), irinotecan(100 mg/kg) for in vivo experiments (i.p) according to manufacturer’s instructions (5-FU, #HY-90006; oxaliplatin, #HY-17371; irinotecan, #HY-16562, MCE).

Techniques: Flow Cytometry, Knockdown, Over Expression

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Solubility of 5-FU determined in different types of oils and surfactants.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Solubility

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: 5-FU-DDTC complex formation by Keto-enol mechanism.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Distribution of 5-FU-NE is determined by dynamic light scattering techniques (A), zeta potential of 5-FU-NE (B), SEM images (C), and TEM images (D).

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Zeta Potential Analyzer

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: DSC-thermograms showed endothermic peaks of castor oil, 5-FU, Transcutol HP, PEG-400, 5-FU-NE, Carbopol and freeze dried optimized-5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Spectral analysis of ATR of castor oil, 5-FU, Transcutol HP, PEG-400, 5-FU-NE, Carbopol and freeze dried optimized-5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: showed the %age-cumulative-release of 5-FU from 5-FU-NE and 5-FU-NE-Gel when it compared with pure 5-FU-S.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: showed a comparative ex vivo various skins (cow, goat, & rat) permeation study of 5-FU from 5-FU-NE and 5-FU-NE-Gel.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Ex Vivo

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: %Age permeation of 5-FU and their retention of skin in stratum corneum (SC) and viable part of the skin ex-vivo from 5-FU, 5-FU-NE, and 5-FU-NE-Gel nanoformulation.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Ex Vivo

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Mass spectrum of 5-FU-MS-Scan i.e. protonated precursor [M-H] – ions at m / z 129 [A], 5-FU-MSMS-Scan fragmentation transitions i.e. major fragmented product ion at m / z 42 [B], and a chromatogram of UHPLC showing retention time 1.710 min.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Mass spectrum of Diethyldithiocarbamate (DDTC)-MS-Scan and Sodium-Diethyldithiocarbamate (DDTC)-MS-Scan showed at protonated precursor [M-H] – ions at m / z 147.27, and 170.21 [A], MS-Scan of complex 5-FU with Diethyldithiocarbamate (DDTC)-MS-Scan and Sodium-Diethyldithiocarbamate (DDTC)-MS-Scan product ion at m / z 277.09 and 300.26 [B], and a UHPLC chromatogram of showing retention time 0.736 and 2.185 min for DDTC chelating agent individual and DDTC–5-FU complex [C].

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: Fluorescence micrographs of cow- and goat-ear pinna skin and rat skin treated with the formulation 5-FU-S (A) 5-FU-NE (B) 5-FU-NE-Gel (C). Different type of formulations labelled with hydrophilic fluorescence probe (6-carboxyfluorescein) to mark the penetration of the 5-FU to deep skin strata.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Fluorescence, Formulation

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: %Age-Cell viability (n = 3, SD, mean) of 5-FU in free 5-FU aqueous solution, control, and optimized-5-FU-NE, and 5-FU-NE-Gel nanoformulation on melanoma cell lines. Optimized–5-FU-NE3 (p < 0.05) and 5-FU-NE3-Gel (p < 0.01) were highly significant than free 5-FU-S.

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: A comparative ex vivo permeation evaluation of a novel 5-Fluorocuracil nanoemulsion-gel by topically applied in the different excised rat, goat, and cow skin

doi: 10.1016/j.sjbs.2020.02.014

Figure Lengend Snippet: IC50 and % Cytotoxicity of free 5-FU, opt-5-FU-NE, 5-FU-NE-Gel, and control on melanoma cancer cell lines (n = 3).

Article Snippet: 5-Fluorouracil was obtained from Chem-Impex International, Inc. Milli-Q-water purification system (ELGA, Made in UK) was used for purification of water i.e. Milli-Q-water.

Techniques: Concentration Assay